Haemoglobin by CMG method

 Aim:

To determine the concentration of hemoglobin in a blood sample using the Cyanmethemoglobin (CMG) method.

Principle:

The CMG method involves converting hemoglobin in the blood sample to cyanmethemoglobin by reacting it with potassium ferricyanide and potassium cyanide. Cyanmethemoglobin has a stable color, allowing for spectrophotometric measurement at a specific wavelength. The absorbance of the resulting solution is proportional to the concentration of hemoglobin present, enabling quantification.

Equipment and Reagents Required:

1. Spectrophotometer

2. Test tubes

3. Pipettes

4. Potassium ferricyanide solution

5. Potassium cyanide solution

6. Blood sample

7. Distilled water

Procedure:

1. Prepare a series of dilutions of the blood sample using distilled water.

2. Mix each diluted blood sample with potassium ferricyanide and potassium cyanide solutions.

3. Incubate the mixture to allow the formation of cyanmethemoglobin.

4. Measure the absorbance of each sample at a specific wavelength using a spectrophotometer.

5. Plot a standard curve using known concentrations of hemoglobin.

6. Determine the concentration of hemoglobin in the original blood sample from the standard curve.

Use:

The CMG method is commonly used in clinical laboratories to measure hemoglobin levels in blood samples. It provides accurate and precise results and is suitable for diagnosing anemia, monitoring response to treatment, and assessing overall health.

Diseases Detected:

Anemia (both iron-deficiency and hemolytic), polycythemia, and other hematological disorders characterized by abnormal hemoglobin levels can be detected using the CMG method.