Total RBC count

-


 Aim

To determine the total number of red blood cells (RBCs) present in a unit volume of blood.


Principle

The principle of total RBC count involves counting the number of red blood cells in a diluted blood sample under a microscope. The dilution factor and the volume of the blood sample are used to calculate the total RBC count.


Equipment and Reagents Required

1. Hemocytometer

2. Microscope

3. Pipettes

4. Diluting fluid (e.g., Hayem's solution)

5. Lancing device or needle

6. Blood collection tube


Procedure

1. Collect a blood sample using a lancing device or needle and anticoagulated blood collection tube.

2. Dilute the blood sample with a known volume of diluting fluid in a specific ratio (commonly 1:200).

3. Mix the diluted blood sample thoroughly to ensure uniform distribution.

4. Transfer a measured volume of the diluted blood sample into the hemocytometer.

5. Allow the red blood cells to settle in the counting chamber.

6. Count the number of red blood cells in multiple fields of view under the microscope.

7. Calculate the average number of RBCs per unit volume of blood using the dilution factor and volume of blood counted.


Use

Total RBC count is a routine hematological test used to assess the oxygen-carrying capacity of the blood and diagnose various disorders such as anemia, polycythemia, and certain hematological malignancies.


Detected Diseases

Anemia (including iron-deficiency anemia, vitamin B12 deficiency anemia, and hemolytic anemia), polycythemia vera, chronic kidney disease, and bone marrow disorders can be detected or monitored using total RBC count.

Comments

Post a Comment

Popular posts from this blog

Sahli's Hemoglobin Method

-
Image
Aim The aim of Sahli's hemoglobin method is to measure the concentration of hemoglobin in a blood sample. Hemoglobin is a protein found in red blood cells that carries oxygen throughout the body. Principle Sahli's hemoglobin method is based on the colorimetric principle. Hemoglobin is converted to acid hematin, which has a brown color. The intensity of the brown color is proportional to the concentration of hemoglobin in the sample. The color of the sample is then compared to a standard color scale to determine the hemoglobin concentration. Equipment and reagents  * Sahli's hemoglobinometer  * Pipettes  * Stirrer  * Hydrochloric acid  * Distilled water Procedure 1. Take a measured volume of blood (usually 20 μL) using a pipette and transfer it into the Sahli's hemoglobinometer. 2. Add distilled water to dilute the blood sample in the hemoglobinometer to a specific mark. 3. Add a few drops of potassium cyanide solution to convert hemoglobin to cyanmethemoglobin, ...
Read more

LME Assignment

-
∆ LME Assignment 1 1) What is GLP? [2] =>Good Laboratory Practice (GLP) is a set of quality assurance principles ensuring the consistency, reliability, and integrity of non-clinical laboratory studies, essential for regulatory compliance in research and development. 2) Discuss the benefits of GLP. [2] =>Good Laboratory Practice (GLP) offers several benefits in research and development: i. Data Integrity: GLP ensures the accuracy, reliability, and consistency of data, fostering confidence in study outcomes and supporting informed decision-making. ii. Regulatory Compliance: Adhering to GLP standards is crucial for regulatory approval, demonstrating that studies are conducted with high quality and in compliance with established guidelines. iii. Traceability and Reproducibility: GLP emphasizes thorough documentation, enabling easy traceability of procedures and facilitating the reproducibility of experiments, enhancing the scientific validity of results. iv. Risk Management: By im...
Read more